Effeminate fifth grader Shuuichi Nitori is considered by most to be one of the prettiest girls in school, but much to her dismay, she is actually biologically male. Fortunately, Shuuichi has a childhood friend who has similar feelings of discomfort related to gender identity: the lanky tomboy Yoshino Takatsuki, who, though biologically female, does not identify as a girl. These two friends share a similar secret and find solace in one another; however, their lives become even more complicated when they must tread the unfamiliar waters of a new school, attempt to make new friends, and struggle to maintain old ones. Faced with nearly insurmountable odds, they must learn to deal with the harsh realities of growing up, being transgender, relationships, and acceptance. Lauded as a decidedly serious take on gender identity and LGBT struggles, Takako Shimura's Hourou Musuko is about Shuuichi and Yoshino's attempts to discover their true selves as they enter puberty, make friends, fall in love, and face some very real and difficult choices.

The full versions of episodes 10 and 11 that were previously edited together into episode 10 of the TV series.

Noya Itsuki just transferred to Ebisugawa High School. Since he has always had a fascination with space, he decides to join the Astronomy Club; however, he mistakenly walks into the Asstronomy Club—a club whose members are all female otaku. Itsuki notices that the members are really weird, but he has yet to realize that he joined the wrong club. His life becomes very strange very quickly.

Blu-ray disc bundled with the fourth volume of the manga.